PerCP-Vio700
PerCP-Vio®770 (Ex-Max 482 nm/Em-Max 704 nm)
PerCP-Vio® 770 is a far-red-emitting tandem fluorophore that combines PerCP and a Vio®770 dye. The donor, PerCP, can be excited by the 488 nm blue laser and transfers energy to the acceptor, Vio®770, which emits light that can be captured with a 780/60 nm bandpass filter. APC-Vio®770 has an excitation peak at 482 nm and an emission peak at 704 nm, and is spectrally similar to PerCP-Cy5.5 and PerCP-eF710. PerCP-Vio®770 is known to be very bright and photostable, as well as being stable when treated with fixatives. These favorable characteristics that make it useful in multiple different applications such as flow cytometry and Fluorescence Microscopy. The large stokes shift can be advantageous when trying to fit more colors into a multicolor panel. The Vio® dye family are products of Miltenyi Biotec, with many antibody conjugates designed and validated for flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 482 nm
- Emission maximum
- 704 nm
- Optimal laser line
- 488
- Optimal filter
- 670/30
- Relative brightness
- 3 of 5
- Commercial conjugates
- 395 from 2 suppliers
PerCP-Vio700 has an excitation maximum at 482 nm and an emission maximum at 704 nm, a Stokes shift of 222 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is PerCP-Vio700?
- PerCP-Vio® 770 is a far-red-emitting tandem fluorophore that combines PerCP and a Vio®770 dye. The donor, PerCP, can be excited by the 488 nm blue laser and transfers energy to the acceptor, Vio®770, which emits light that can be captured with a 780/60 nm bandpass filter. APC-Vio®770 has an excitation peak at 482 nm and an emission peak at 704 nm, and is spectrally similar to PerCP-Cy5.5 and PerCP-eF710. PerCP-Vio®770 is known to be very bright and photostable, as well as being stable when treated with fixatives. These favorable characteristics that make it useful in multiple different applications such as flow cytometry and Fluorescence Microscopy. The large stokes shift can be advantageous when trying to fit more colors into a multicolor panel. The Vio® dye family are products of Miltenyi Biotec, with many antibody conjugates designed and validated for flow cytometry.
- What laser excites PerCP-Vio700?
- PerCP-Vio700 is optimally excited by the 488 laser line. Its excitation maximum is 482 nm.
- What filter should I use to detect PerCP-Vio700?
- A 670/30 filter captures the peak of the PerCP-Vio700 emission near 704 nm with minimal spillover.
- How bright is PerCP-Vio700?
- PerCP-Vio700 has a relative brightness of 3 out of 5.
- How many antibodies are available conjugated to PerCP-Vio700?
- FluoroFinder tracks 395 commercially available PerCP-Vio700 conjugates, searchable by target, clone, host and application.