640

Laser Line

780/60

Common Filter

652

Excitation Max

775

Emission Max

1,489

Antibodies


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About APC-Vio770


APC-Vio® 770 is a near infrared-emitting tandem fluorophore that combines allopycocyanin (APC) and a Vio770 dye. The donor, APC, can be excited by the 640 nm red laser and transfers energy to the acceptor, Vio770, which emits light that can be captured with a 780/60 nm bandpass filter. APC-Vio770 has an excitation peak at 652 nm and an emission peak at 775 nm, and is spectrally similar to APC-CF®770 (Biotium), APC-Cy® 7 (GE Healthcare) APC-H7 (BD Biosciences), APC-eFluor™ 780 (ThermoFisher Scientific), APC-Vio®770 (Miltenyi Biotec), APC-Fire™ 750 (BioLegend) and APC-Fire™ 810 (BioLegend). APC-Vio770 is mainly used for flow cytometry and is often a good option because it emits brightly in the near infrared end of the spectrum and has very little spillover into other commonly used detection channels. The large stokes shift can be advantageous when trying to fit more colors into a multicolor panel. The Vio® dye family are products of Miltenyi Biotec, with many antibody conjugates designed and validated for flow cytometry.


Popular APC-Vio770 Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
Miltenyi Biotec
130-110-522
FC CD14
Human
cell line
IgG1
REA599
100 Tests in 200 μl
$340
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Miltenyi Biotec
130-110-681
FC CD8
Human
cell line
IgG1
REA734
100 Tests in 200 μl
$340
View
Miltenyi Biotec
130-112-650
FC CD62L
Mouse
cell line
IgG1
REA828
150 μg in 1 ml
$435
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Miltenyi Biotec
130-111-919
FC Ly-6C
Mouse
cell line
IgG1
REA796
30 μg in 200 μl
$151
View
Miltenyi Biotec
130-113-773
FC CD4
Human
Mouse
IgG2a κ
VIT4
30 Tests in 60 μl
$149
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Miltenyi Biotec
130-113-136
FC CD3
Human
cell line
IgG1
REA613
100 Tests in 200 μl
$340
View
Miltenyi Biotec
130-113-416
FC CD127
Human
cell line
IgG1
REA614
100 Tests in 200 μl
$351
View
Miltenyi Biotec
130-120-737
FC CD8a
Mouse
cell line
IgG1
REA601
150 μg in 1 ml
$372
View
Miltenyi Biotec
130-120-438
FC TCR V delta 1
Human
cell line
IgG1
REA173
100 Tests in 200 μl
$340
View
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