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DiBAC4(3)
DiBAC4(3) (bis-(1,3-dibutylbarbituric acid)trimethine oxonol) is a slow-response, anionic membrane potential dye that partitions into depolarized cells, making it a popular tool for monitoring changes in plasma membrane potential by flow cytometry or fluorescence microscopy. It's peak excitation is 494 nm and peak emission is at 517 nm, spectrally similar to FITC.
Excitation & emission spectra
Key facts
- Excitation maximum
- 494 nm
- Emission maximum
- 517 nm
- Optimal filter
- 515/30
- Commercial antibodies
- 1 from 1 supplier
DiBAC4(3) has an excitation maximum at 494 nm and an emission maximum at 517 nm, a Stokes shift of 23 nm.
Frequently asked questions
- What is DiBAC4(3)?
- DiBAC4(3) (bis-(1,3-dibutylbarbituric acid)trimethine oxonol) is a slow-response, anionic membrane potential dye that partitions into depolarized cells, making it a popular tool for monitoring changes in plasma membrane potential by flow cytometry or fluorescence microscopy. It's peak excitation is 494 nm and peak emission is at 517 nm, spectrally similar to FITC.
- What filter should I use to detect DiBAC4(3)?
- A 515/30 filter captures the peak of the DiBAC4(3) emission near 517 nm with minimal spillover.
- How many antibodies are available conjugated to DiBAC4(3)?
- FluoroFinder tracks 1 commercially available DiBAC4(3) conjugates, searchable by target, clone, host and application.