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iFluor 440
iFluor® 440 from AAT Bioquest is a fluorophore with an excitation peak at 434 nm and an emission peak at 480 nm. It can be useful in both microscopy and flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 434 nm
- Emission maximum
- 480 nm
- Optimal laser line
- 405
- Relative brightness
- 3 of 5
- Extinction coefficient
- 40,000 cm⁻¹ M⁻¹
- Quantum yield
- 0.67
iFluor 440 has an excitation maximum at 434 nm and an emission maximum at 480 nm, a Stokes shift of 46 nm. Its extinction coefficient is 40,000 cm⁻¹ M⁻¹ M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is iFluor 440?
- iFluor® 440 from AAT Bioquest is a fluorophore with an excitation peak at 434 nm and an emission peak at 480 nm. It can be useful in both microscopy and flow cytometry.
- What laser excites iFluor 440?
- iFluor 440 is optimally excited by the 405 laser line. Its excitation maximum is 434 nm.
- How bright is iFluor 440?
- iFluor 440 has a relative brightness of 3 out of 5 (quantum yield 0.67).