What laser excites Spark PLUS UV395?

Spark PLUS UV395 is optimally excited by the 355 nm laser line. Its excitation maximum is 355 nm.

What filter should I use to detect Spark PLUS UV395?

A 379/28 filter captures the peak of the Spark PLUS UV395 emission near 385 nm with minimal spillover.

How bright is Spark PLUS UV395?

Spark PLUS UV395 has a relative brightness of 4 out of 5.

How many antibodies are available conjugated to Spark PLUS UV395?

FluoroFinder tracks 410 commercially available Spark PLUS UV395 conjugates, searchable by target, clone, host and application.

Spark PLUS UV395

Spark PLUS UV395™ has an excitation peak at 355 nm and an emission peak at 385 nm. Spark PLUS UV395™ is the first in this new family of Spark Dyes formulated to advance applications in multicolor flow cytometry. Spark PLUS Dyes employ proprietary technology to maximize signal and boost performance while maintaining the advantages of Spark Dyes, such as high stability and buffer compatibility. Spark PLUS UV395™ is designed to be excited by the UV laser, emitting a spectrum that most closely matches BD Horizon™ BUV395. This dye is useful for both conventional and spectral flow cytometry, and its intense brightness makes it a versatile option for detecting antigens at a variety of expression levels. Spark PLUS UV395™ requires no special buffers for staining and can be unmixed from Spark UV 387 on spectral cytometers with careful panel design.

Excitation & emission spectra

Key facts

Excitation maximum: 355 nm
Emission maximum: 385 nm
Optimal laser line: 355 nm
Optimal filter: 379/28
Relative brightness: 4 of 5
Commercial antibodies: 410 from 2 suppliers

Spark PLUS UV395 has an excitation maximum at 355 nm and an emission maximum at 385 nm, a Stokes shift of 30 nm. It is optimally excited by the 355 nm laser line.

Frequently asked questions

What is Spark PLUS UV395?: Spark PLUS UV395™ has an excitation peak at 355 nm and an emission peak at 385 nm. Spark PLUS UV395™ is the first in this new family of Spark Dyes formulated to advance applications in multicolor flow cytometry. Spark PLUS Dyes employ proprietary technology to maximize signal and boost performance while maintaining the advantages of Spark Dyes, such as high stability and buffer compatibility. Spark PLUS UV395™ is designed to be excited by the UV laser, emitting a spectrum that most closely matches BD Horizon™ BUV395. This dye is useful for both conventional and spectral flow cytometry, and its intense brightness makes it a versatile option for detecting antigens at a variety of expression levels. Spark PLUS UV395™ requires no special buffers for staining and can be unmixed from Spark UV 387 on spectral cytometers with careful panel design.
What laser excites Spark PLUS UV395?: Spark PLUS UV395 is optimally excited by the 355 nm laser line. Its excitation maximum is 355 nm.
What filter should I use to detect Spark PLUS UV395?: A 379/28 filter captures the peak of the Spark PLUS UV395 emission near 385 nm with minimal spillover.
How bright is Spark PLUS UV395?: Spark PLUS UV395 has a relative brightness of 4 out of 5.
How many antibodies are available conjugated to Spark PLUS UV395?: FluoroFinder tracks 410 commercially available Spark PLUS UV395 conjugates, searchable by target, clone, host and application.