RealBlue 705
RB705
RealBlue™ 705 from BD Biosciences is a novel fluorescent multimer that exhibits improvements upon PerCP-Cy5.5 and BB700 due to lower cross beam excitation efficiency with the 355nm UV, 405nm violet, 561nm yellow/green and 633/647nm red lasers, which results in less spectral spillover and resultant spreading error associated with the use of this fluorophore in a multicolor panel.
Excitation & emission spectra
Key facts
- Excitation maximum
- 498 nm
- Emission maximum
- 707 nm
- Optimal laser line
- 488
- Relative brightness
- 5 of 5
- Commercial conjugates
- 10 from 1 supplier
RealBlue 705 has an excitation maximum at 498 nm and an emission maximum at 707 nm, a Stokes shift of 209 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is RealBlue 705?
- RealBlue™ 705 from BD Biosciences is a novel fluorescent multimer that exhibits improvements upon PerCP-Cy5.5 and BB700 due to lower cross beam excitation efficiency with the 355nm UV, 405nm violet, 561nm yellow/green and 633/647nm red lasers, which results in less spectral spillover and resultant spreading error associated with the use of this fluorophore in a multicolor panel.
- What laser excites RealBlue 705?
- RealBlue 705 is optimally excited by the 488 laser line. Its excitation maximum is 498 nm.
- How bright is RealBlue 705?
- RealBlue 705 has a relative brightness of 5 out of 5.
- How many antibodies are available conjugated to RealBlue 705?
- FluoroFinder tracks 10 commercially available RealBlue 705 conjugates, searchable by target, clone, host and application.