About Spark Red 718

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Spark Red™ 718 is a brighter alternative to Alexa Fluor® 700, adding to the fluorophore options for the red laser that can detect antigens with lower expression levels. It can be used on a conventional cytometer with a 633 nm red laser and 720/45 filter set (or equivalent filter used to detect Alexa Fluor® 700) spectral cytometers with appropriate instrument settings. On conventional cytometers, Spark Red™ 718 can be utilized with both APC and APC/Cyanine7. On spectral cytometers, it can be unmixed from APC, Spark NIR™ 685, Alexa Fluor® 660, APC/Cyanine7, and APC/Fire™ 810. As spectral properties of Spark Red™ 718 and Alexa Fluor® 700 are nearly identical, using them together in a multicolor panel is not recommended. With a medium brightness, Spark Red™ 718 is best suited for detecting antigens with low to moderate expression levels.

Dyes similar to Spark Red 718 based on emission

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Alexa Fluor 680 APC-Alexa 680 APC-Alexa 700 APC-R700 ATTO 700 Brilliant Violet 711 CellBrite Steady 685 CellVue Burgundy CF680R DyLight 680 Ghost Dye Red 710 iFluor 680 iFluor 700 IFP 1.4 iRFP 702 iRFP LDS 751 Live/Dead Fix Scarlet LysoView 680 MemBrite Fix 680/700 NeuroVue Burgundy NovaFluor Red 710 NovaFluor Yellow 730 NucSpot 680 PE-Alexa 700 PerCP-eFluor 710 PerCP-Vio700 PromoFluor 680 Qdot 705 ReadiLink 680-701 ReadiLink 700-713 Red 718 redFluor 710 Seta-APC-705 SeTau-665-NHS SeTau-680-NHS StarBright Violet 710 StarBright Yellow 720 SuperBright 702 SureLight PerCP Cyanine 5.5 CytoLiner 680/710 RealBlue 705 ABflo 700 ABflo 680 Aluora 700 XFD680 RealYellow 703 AbBy Fluor 680 XFD700 RayBright Red 700 NucSpot 680/700 Alexa Fluor Plus 680 APC Far Red 718 BactoView Dead 690/710