ViaFluor 647
ViaFluor 647 (Ex-Max 655 nm/Em-Max 681 nm)
ViaFluor 647 is a red fluorescent dye used to stain microtubules in live cells. It can be used in live-cell Fluorescence Microscopy applications such as Fluorescence Microscopy and super resolution imaging by STED.
Excitation & emission spectra
Key facts
- Excitation maximum
- 655 nm
- Emission maximum
- 681 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
- Relative brightness
- 4 of 5
ViaFluor 647 has an excitation maximum at 655 nm and an emission maximum at 681 nm, a Stokes shift of 26 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is ViaFluor 647?
- ViaFluor 647 is a red fluorescent dye used to stain microtubules in live cells. It can be used in live-cell Fluorescence Microscopy applications such as Fluorescence Microscopy and super resolution imaging by STED.
- What laser excites ViaFluor 647?
- ViaFluor 647 is optimally excited by the 628-640 laser line. Its excitation maximum is 655 nm.
- What filter should I use to detect ViaFluor 647?
- A 695/40 filter captures the peak of the ViaFluor 647 emission near 681 nm with minimal spillover.
- How bright is ViaFluor 647?
- ViaFluor 647 has a relative brightness of 4 out of 5.