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CF754

CF®754 (Ex-Max 748 nm/Em-Max 793 nm)

CF®754 from Biotium is a fluorophore with an excitation peak at 748 nm and an emission peak at 793 nm. CF®750 has been specifically validated in super-resolution imaging by STORM. It is spectrally similar to Alexa Fluor™ 750 and Cy7.

Excitation & emission spectra

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Key facts

Excitation maximum
748 nm
Emission maximum
793 nm
Optimal laser line
730-750
Optimal filter
780/60

CF754 has an excitation maximum at 748 nm and an emission maximum at 793 nm, a Stokes shift of 45 nm. It is optimally excited by the 730-750 laser line.

Frequently asked questions

What is CF754?
CF®754 from Biotium is a fluorophore with an excitation peak at 748 nm and an emission peak at 793 nm. CF®750 has been specifically validated in super-resolution imaging by STORM. It is spectrally similar to Alexa Fluor™ 750 and Cy7.
What laser excites CF754?
CF754 is optimally excited by the 730-750 laser line. Its excitation maximum is 748 nm.
What filter should I use to detect CF754?
A 780/60 filter captures the peak of the CF754 emission near 793 nm with minimal spillover.