Live/Dead Fix Scarlet
Live/Dead Fix Scarlet (Ex-Max 490 nm/Em-Max 510 nm)
The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Scarlet has an excitation peak at 694 nm and an emission peak at 715 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 694 nm
- Emission maximum
- 715 nm
- Optimal laser line
- 638
- Optimal filter
- 690/45
Live/Dead Fix Scarlet has an excitation maximum at 694 nm and an emission maximum at 715 nm, a Stokes shift of 21 nm. It is optimally excited by the 638 laser line.
Frequently asked questions
- What is Live/Dead Fix Scarlet?
- The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Scarlet has an excitation peak at 694 nm and an emission peak at 715 nm.
- What laser excites Live/Dead Fix Scarlet?
- Live/Dead Fix Scarlet is optimally excited by the 638 laser line. Its excitation maximum is 694 nm.
- What filter should I use to detect Live/Dead Fix Scarlet?
- A 690/45 filter captures the peak of the Live/Dead Fix Scarlet emission near 715 nm with minimal spillover.