Live-or-Dye 330/410
Live-or-Dye 330/410 (Ex-Max 330 nm/Em-Max 410 nm)
Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 330/410 has an excitation peak at 330 nm and an emission peak at 410 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 330 nm
- Emission maximum
- 410 nm
- Optimal laser line
- 355
- Optimal filter
- 379/28
- Commercial conjugates
- 2 from 1 supplier
Live-or-Dye 330/410 has an excitation maximum at 330 nm and an emission maximum at 410 nm, a Stokes shift of 80 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is Live-or-Dye 330/410?
- Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 330/410 has an excitation peak at 330 nm and an emission peak at 410 nm.
- What laser excites Live-or-Dye 330/410?
- Live-or-Dye 330/410 is optimally excited by the 355 laser line. Its excitation maximum is 330 nm.
- What filter should I use to detect Live-or-Dye 330/410?
- A 379/28 filter captures the peak of the Live-or-Dye 330/410 emission near 410 nm with minimal spillover.
- How many antibodies are available conjugated to Live-or-Dye 330/410?
- FluoroFinder tracks 2 commercially available Live-or-Dye 330/410 conjugates, searchable by target, clone, host and application.