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SureLight PerCP Cyanine 5.5

SureLight PerCP Cyanine 5.5 (Ex-Max 483 nm/Em-Max 702 nm)

SureLight PerCP-Cyanine 5.5 from Columbia Biosciences is a tandem fluorophore with an excitation peak at 483 nm and an emission peak at 702 nm. SureLight PerCP-Cyanine 5.5 is available in antibody conjugation kits for use in flow cytometry.

Excitation & emission spectra

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Key facts

Excitation maximum
483 nm
Emission maximum
702 nm
Optimal laser line
488
Optimal filter
695/40
Relative brightness
2 of 5

SureLight PerCP Cyanine 5.5 has an excitation maximum at 483 nm and an emission maximum at 702 nm, a Stokes shift of 219 nm. It is optimally excited by the 488 laser line.

Frequently asked questions

What is SureLight PerCP Cyanine 5.5?
SureLight PerCP-Cyanine 5.5 from Columbia Biosciences is a tandem fluorophore with an excitation peak at 483 nm and an emission peak at 702 nm. SureLight PerCP-Cyanine 5.5 is available in antibody conjugation kits for use in flow cytometry.
What laser excites SureLight PerCP Cyanine 5.5?
SureLight PerCP Cyanine 5.5 is optimally excited by the 488 laser line. Its excitation maximum is 483 nm.
What filter should I use to detect SureLight PerCP Cyanine 5.5?
A 695/40 filter captures the peak of the SureLight PerCP Cyanine 5.5 emission near 702 nm with minimal spillover.
How bright is SureLight PerCP Cyanine 5.5?
SureLight PerCP Cyanine 5.5 has a relative brightness of 2 out of 5.