Vio R720
Vio® R720 (Ex-Max 695 nm/Em-Max 720 nm)
Vio® Bright R720 and Vio® R720 have an excitation peak at 695 nm and an emission peak at 720 nm, which means that these dyes are optimally excited by the 640 nm red laser while the emitted light is collected by the channel with a 725/40 bandpass filter. Miltenyi Biotec's proprietary multimerization technology allows for Vio® Bright R720 to be nearly twice the brightness of Vio® R720, so this choice of fluorophore offers flexibility in your panel design in flow cytometry. Spectrally, Vio® Bright R720 is comparable to R700 from BD Biosciences.
Excitation & emission spectra
Key facts
- Excitation maximum
- 695 nm
- Emission maximum
- 720 nm
- Optimal laser line
- 628-640
- Optimal filter
- 725/40
- Relative brightness
- 2 of 5
- Commercial antibodies
- 7 from 1 supplier
Vio R720 has an excitation maximum at 695 nm and an emission maximum at 720 nm, a Stokes shift of 25 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Vio R720?
- Vio® Bright R720 and Vio® R720 have an excitation peak at 695 nm and an emission peak at 720 nm, which means that these dyes are optimally excited by the 640 nm red laser while the emitted light is collected by the channel with a 725/40 bandpass filter. Miltenyi Biotec's proprietary multimerization technology allows for Vio® Bright R720 to be nearly twice the brightness of Vio® R720, so this choice of fluorophore offers flexibility in your panel design in flow cytometry. Spectrally, Vio® Bright R720 is comparable to R700 from BD Biosciences.
- What laser excites Vio R720?
- Vio R720 is optimally excited by the 628-640 laser line. Its excitation maximum is 695 nm.
- What filter should I use to detect Vio R720?
- A 725/40 filter captures the peak of the Vio R720 emission near 720 nm with minimal spillover.
- How bright is Vio R720?
- Vio R720 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to Vio R720?
- FluoroFinder tracks 7 commercially available Vio R720 conjugates, searchable by target, clone, host and application.