CF583R
CF®583R (Ex-Max 587 nm/Em-Max 608 nm)
CF®583R from Biotium is a fluorophore with an excitation peak at 583 nm and an emission peak at 606 nm. It can be useful in both fluorescence microscopy and spectral flow cytometry but was designed for use in super-resolution imaging using STORM.
Excitation & emission spectra
Key facts
- Excitation maximum
- 587 nm
- Emission maximum
- 608 nm
- Optimal laser line
- 561
- Optimal filter
- 610/20
- Relative brightness
- 2 of 5
- Extinction coefficient
- 100000
- Molecular weight
- 773
- Commercial conjugates
- 6 from 1 supplier
CF583R has an excitation maximum at 587 nm and an emission maximum at 608 nm, a Stokes shift of 21 nm. Its extinction coefficient is 100000 M⁻¹cm⁻¹. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is CF583R?
- CF®583R from Biotium is a fluorophore with an excitation peak at 583 nm and an emission peak at 606 nm. It can be useful in both fluorescence microscopy and spectral flow cytometry but was designed for use in super-resolution imaging using STORM.
- What laser excites CF583R?
- CF583R is optimally excited by the 561 laser line. Its excitation maximum is 587 nm.
- What filter should I use to detect CF583R?
- A 610/20 filter captures the peak of the CF583R emission near 608 nm with minimal spillover.
- How bright is CF583R?
- CF583R has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to CF583R?
- FluoroFinder tracks 6 commercially available CF583R conjugates, searchable by target, clone, host and application.