CF680T
CF®680T (Ex-Max 681 nm/Em-Max 698 nm)
CF®680T from Biotium is a fluorophore with an excitation peak at 681 nm and an emission peak at 698nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 680, IRDye® 680 and Cy5.5.
Excitation & emission spectra
Key facts
- Excitation maximum
- 681 nm
- Emission maximum
- 698 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
- Relative brightness
- 1 of 5
CF680T has an excitation maximum at 681 nm and an emission maximum at 698 nm, a Stokes shift of 17 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is CF680T?
- CF®680T from Biotium is a fluorophore with an excitation peak at 681 nm and an emission peak at 698nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 680, IRDye® 680 and Cy5.5.
- What laser excites CF680T?
- CF680T is optimally excited by the 628-640 laser line. Its excitation maximum is 681 nm.
- What filter should I use to detect CF680T?
- A 695/40 filter captures the peak of the CF680T emission near 698 nm with minimal spillover.
- How bright is CF680T?
- CF680T has a relative brightness of 1 out of 5.