MemBrite Fix 660/680
MemBrite Fix 660/680 (Ex-Max 662 nm/Em-Max 682 nm)
Biotium's MemBrite Fix and Membrite Fix-ST dyes are amine-reactive cell surface staining dyes that conjugate cell surface proteins to stain the outer membrane of live cells. A great benefit to these dyes is that MemBrite Fix dyes can withstand fixation and permeabilization which allows extracellular staining to be combined with intracellular staining techniques. Additionally, the MemBrite Fix-ST dyes (and MemBrite Fix 680/700) are compatible with super resolution microscopy and Fluorescence Microscopy techniques such as STORM. MemBrite Fix 660/680 has an excitaion peak at 662 nm and an emission peak at 682 nm and are recommended for use in fluorescence microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 662 nm
- Emission maximum
- 682 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
MemBrite Fix 660/680 has an excitation maximum at 662 nm and an emission maximum at 682 nm, a Stokes shift of 20 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is MemBrite Fix 660/680?
- Biotium's MemBrite Fix and Membrite Fix-ST dyes are amine-reactive cell surface staining dyes that conjugate cell surface proteins to stain the outer membrane of live cells. A great benefit to these dyes is that MemBrite Fix dyes can withstand fixation and permeabilization which allows extracellular staining to be combined with intracellular staining techniques. Additionally, the MemBrite Fix-ST dyes (and MemBrite Fix 680/700) are compatible with super resolution microscopy and Fluorescence Microscopy techniques such as STORM. MemBrite Fix 660/680 has an excitaion peak at 662 nm and an emission peak at 682 nm and are recommended for use in fluorescence microscopy.
- What laser excites MemBrite Fix 660/680?
- MemBrite Fix 660/680 is optimally excited by the 628-640 laser line. Its excitation maximum is 662 nm.
- What filter should I use to detect MemBrite Fix 660/680?
- A 695/40 filter captures the peak of the MemBrite Fix 660/680 emission near 682 nm with minimal spillover.