PE-Fire 700
PE-Fire™ 700 (Ex-Max 565 nm/Em-Max 695 nm)
PE-Fire™ 700 from Biolegend is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Fire™ 700. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Fire™ 700. This tandem has an excitation peak at 565 nm and an emission peak at 695 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 565 nm
- Emission maximum
- 695 nm
- Optimal laser line
- 488-561
- Optimal filter
- 695/40
- Relative brightness
- 5 of 5
- Commercial conjugates
- 21 from 2 suppliers
PE-Fire 700 has an excitation maximum at 565 nm and an emission maximum at 695 nm, a Stokes shift of 130 nm. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is PE-Fire 700?
- PE-Fire™ 700 from Biolegend is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Fire™ 700. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Fire™ 700. This tandem has an excitation peak at 565 nm and an emission peak at 695 nm.
- What laser excites PE-Fire 700?
- PE-Fire 700 is optimally excited by the 488-561 laser line. Its excitation maximum is 565 nm.
- What filter should I use to detect PE-Fire 700?
- A 695/40 filter captures the peak of the PE-Fire 700 emission near 695 nm with minimal spillover.
- How bright is PE-Fire 700?
- PE-Fire 700 has a relative brightness of 5 out of 5.
- How many antibodies are available conjugated to PE-Fire 700?
- FluoroFinder tracks 21 commercially available PE-Fire 700 conjugates, searchable by target, clone, host and application.