640

Laser Line

780/60

Common Filter

650

Excitation Max

807

Emission Max

142

Antibodies


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About APC-Fire 810


APC-Fire™ 810 is an infrared-emitting tandem fluorophore that combines allophycocyanin (APC) and Fire 810. The donor, APC, can be excited by the 640 nm red laser and transfers energy to the acceptor, Fire 810, which emits light that can be captured using a 780/60 bandpass filter. APC/Fire 810 has an excitation peak at 650nm and an emission peak at 807 nm, and is spectrally similar to APC-CF®770 (Biotium), APC-Cy® 7 (GE Healthcare) APC-H7 (BD Biosciences), APC-eFluor™ 780 (ThermoFisher Scientific), APC-Vio®770 (Miltenyi Biotec), APC-Fire™ 750 (BioLegend) and APC-Fire™ 810 (BioLegend). APC-Fire 810 is most commonly used in flow cytometry. On a 5-laser Cytek® Aurora, it has very limited spectral spillover into any other channel, so it can be assigned to any antigen with moderate to abundant expression and used in combination with any other fluorophore. This dye is very useful for detecting variably expressed markers, like markers of activation, since it emits so far outside of the autofluorescence range. The Fire™ tandem fluorophore dye line is developed and sold by BioLegend.


Popular APC-Fire 810 Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
BioLegend
344662
FC CD4
Human
Mouse
IgG1 κ
SK3
100 Tests
$413
View
BioLegend
344858
FC CD3
Human,Chimpanzee
Mouse
IgG1 κ
SK7
100 Tests
$413
View
BioLegend
103174
FC CD45
Mouse
Rat
IgG2b κ
30-F11
100 μg
$358
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BioLegend
304076
FC CD45
Human,Chimpanzee
Mouse
IgG1 κ
HI30
100 Tests
$358
View
BioLegend
344764
FC CD8a
Human,African Green Monkey,Chimpanzee,Cynomolgus,Pigtailed Macaque,Rhesus,Non-Human Primate
Mouse
IgG1 κ
SK1
100 Tests
$385
View
BioLegend
100479
FC CD4
Mouse
Rat
IgG2b κ
GK1.5
25 μg
$121
View
BioLegend
100267
FC CD3
Mouse
Rat
IgG2b κ
17A2
25 μg
$121
View
BioLegend
302272
FC CD19
Human,Chimpanzee,Rhesus
Mouse
IgG1 κ
HIB19
100 Tests
$352
View
BioLegend
367732
FC KLRG1
Human
Mouse
IgG2a κ
SA231A2
100 Tests
$385
View
BioLegend
155321
FC CD163
Mouse
Rat
IgG2a κ
S15049I
25 μg
$165
View
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