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Seta-680-NHS

Seta-680-NHS (Ex-Max 678 nm/Em-Max 698 nm)

Seta-680 from SETA BioMedicals is a fluorophore with an excitation peak at 678 nm and an emission peak at 698 nm. Seta dyes are available as streptavidin conjugates or as various reactive dyes for your own conjugation.

Excitation & emission spectra

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Key facts

Excitation maximum
678 nm
Emission maximum
698 nm
Optimal laser line
628-640
Optimal filter
695/40
Relative brightness
2 of 5
Extinction coefficient
200000
Quantum yield
0.24

Seta-680-NHS has an excitation maximum at 678 nm and an emission maximum at 698 nm, a Stokes shift of 20 nm. Its extinction coefficient is 200000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.

Frequently asked questions

What is Seta-680-NHS?
Seta-680 from SETA BioMedicals is a fluorophore with an excitation peak at 678 nm and an emission peak at 698 nm. Seta dyes are available as streptavidin conjugates or as various reactive dyes for your own conjugation.
What laser excites Seta-680-NHS?
Seta-680-NHS is optimally excited by the 628-640 laser line. Its excitation maximum is 678 nm.
What filter should I use to detect Seta-680-NHS?
A 695/40 filter captures the peak of the Seta-680-NHS emission near 698 nm with minimal spillover.
How bright is Seta-680-NHS?
Seta-680-NHS has a relative brightness of 2 out of 5 (quantum yield 0.24).