Seta-385-NHS
Seta-385-NHS (Ex-Max 384 nm/Em-Max 512 nm)
Seta-385 from SETA BioMedicals is a fluorophore with an excitation peak at 384 nm and an emission peak at 512 nm. Seta dyes are available as streptavidin conjugates or as various reactive dyes for your own conjugation.
Excitation & emission spectra
Key facts
- Excitation maximum
- 384 nm
- Emission maximum
- 512 nm
- Optimal laser line
- 405
- Optimal filter
- 515/30
- Relative brightness
- 2 of 5
- Extinction coefficient
- 25000
- Quantum yield
- 0.78
Seta-385-NHS has an excitation maximum at 384 nm and an emission maximum at 512 nm, a Stokes shift of 128 nm. Its extinction coefficient is 25000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Seta-385-NHS?
- Seta-385 from SETA BioMedicals is a fluorophore with an excitation peak at 384 nm and an emission peak at 512 nm. Seta dyes are available as streptavidin conjugates or as various reactive dyes for your own conjugation.
- What laser excites Seta-385-NHS?
- Seta-385-NHS is optimally excited by the 405 laser line. Its excitation maximum is 384 nm.
- What filter should I use to detect Seta-385-NHS?
- A 515/30 filter captures the peak of the Seta-385-NHS emission near 512 nm with minimal spillover.
- How bright is Seta-385-NHS?
- Seta-385-NHS has a relative brightness of 2 out of 5 (quantum yield 0.78).