Loading the Dye Directory…

SureLight PE Cyanine 5.5

SureLight PE-695 (Ex-Max 565 nm/Em-Max 695 nm)

SureLight PE-695 (PE-Cyanine 5.5) from Columbia Biosciences is a tandem with an excitation peak at 488 and 565 nm and an emission peak at 695 nm. SureLight PE-695 is available in antibody conjugation kits for use in flow cytometry.

Excitation & emission spectra

400450500550600650700750800

Key facts

Excitation maximum
565 nm
Emission maximum
695 nm
Optimal laser line
488-561
Optimal filter
695/40
Relative brightness
4 of 5
Molecular weight
250000

SureLight PE Cyanine 5.5 has an excitation maximum at 565 nm and an emission maximum at 695 nm, a Stokes shift of 130 nm. It is optimally excited by the 488-561 laser line.

Frequently asked questions

What is SureLight PE Cyanine 5.5?
SureLight PE-695 (PE-Cyanine 5.5) from Columbia Biosciences is a tandem with an excitation peak at 488 and 565 nm and an emission peak at 695 nm. SureLight PE-695 is available in antibody conjugation kits for use in flow cytometry.
What laser excites SureLight PE Cyanine 5.5?
SureLight PE Cyanine 5.5 is optimally excited by the 488-561 laser line. Its excitation maximum is 565 nm.
What filter should I use to detect SureLight PE Cyanine 5.5?
A 695/40 filter captures the peak of the SureLight PE Cyanine 5.5 emission near 695 nm with minimal spillover.
How bright is SureLight PE Cyanine 5.5?
SureLight PE Cyanine 5.5 has a relative brightness of 4 out of 5.