iRFP 702
iRFP 702 (Ex-Max 673 nm/Em-Max 702 nm)
iRFP702 is a dimeric fluorescent protein derived from the proteobacteria, Rhodopseudomonas palustris. It has an excitation peak at 673 nm and an emission peak at 702 nm. It requires the cofactor biliverdin for fluorescence.
Excitation & emission spectra
Key facts
- Excitation maximum
- 673 nm
- Emission maximum
- 702 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
- Extinction coefficient
- 93000
- Quantum yield
- 0.08
- Molecular weight
- 34500
iRFP 702 has an excitation maximum at 673 nm and an emission maximum at 702 nm, a Stokes shift of 29 nm. Its extinction coefficient is 93000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is iRFP 702?
- iRFP702 is a dimeric fluorescent protein derived from the proteobacteria, Rhodopseudomonas palustris. It has an excitation peak at 673 nm and an emission peak at 702 nm. It requires the cofactor biliverdin for fluorescence.
- What laser excites iRFP 702?
- iRFP 702 is optimally excited by the 628-640 laser line. Its excitation maximum is 673 nm.
- What filter should I use to detect iRFP 702?
- A 695/40 filter captures the peak of the iRFP 702 emission near 702 nm with minimal spillover.