Alexa Fluor 680
Alexa Fluor™ 680 (Ex-Max 679 nm/Em-Max 702 nm)
Alexa Fluor™ 680 (AF680, Alexa 680) has an excitation peak at 679 nm and an emission peak at 702 nm, and is spectrally similar to Cy®5.5 (GE Healthcare), IR680® (Licor), iFluor® 680 (ATT Bioquest) and iFluor® 700 (ATT Bioquest). Alexa 680 is commonly used for flow cytometry, fluorescence microscopy and super-resolution microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 679 nm
- Emission maximum
- 702 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
- Relative brightness
- 1 of 5
- Extinction coefficient
- 184000
- Quantum yield
- 0.36
- Commercial antibodies
- 6.9k from 8 suppliers
Alexa Fluor 680 has an excitation maximum at 679 nm and an emission maximum at 702 nm, a Stokes shift of 23 nm. Its extinction coefficient is 184000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Alexa Fluor 680?
- Alexa Fluor™ 680 (AF680, Alexa 680) has an excitation peak at 679 nm and an emission peak at 702 nm, and is spectrally similar to Cy®5.5 (GE Healthcare), IR680® (Licor), iFluor® 680 (ATT Bioquest) and iFluor® 700 (ATT Bioquest). Alexa 680 is commonly used for flow cytometry, fluorescence microscopy and super-resolution microscopy.
- What laser excites Alexa Fluor 680?
- Alexa Fluor 680 is optimally excited by the 628-640 laser line. Its excitation maximum is 679 nm.
- What filter should I use to detect Alexa Fluor 680?
- A 695/40 filter captures the peak of the Alexa Fluor 680 emission near 702 nm with minimal spillover.
- How bright is Alexa Fluor 680?
- Alexa Fluor 680 has a relative brightness of 1 out of 5 (quantum yield 0.36).
- How many antibodies are available conjugated to Alexa Fluor 680?
- FluoroFinder tracks 6898 commercially available Alexa Fluor 680 conjugates, searchable by target, clone, host and application.