RedDot 1
RedDot 1 (Ex-Max 662 nm/Em-Max 694 nm)
RedDot 1 is a cell-permeant, fluorogenic nucleic acid stain similar to DRAQ5. It has an excitation peak at 662 nm and an emission peak at 694 nm. It can be used with the following analysis platforms: Flow Cytometry and Fluorescence Microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 662 nm
- Emission maximum
- 694 nm
- Optimal laser line
- 561-594
- Optimal filter
- 695/40
RedDot 1 has an excitation maximum at 662 nm and an emission maximum at 694 nm, a Stokes shift of 32 nm. It is optimally excited by the 561-594 laser line.
Frequently asked questions
- What is RedDot 1?
- RedDot 1 is a cell-permeant, fluorogenic nucleic acid stain similar to DRAQ5. It has an excitation peak at 662 nm and an emission peak at 694 nm. It can be used with the following analysis platforms: Flow Cytometry and Fluorescence Microscopy.
- What laser excites RedDot 1?
- RedDot 1 is optimally excited by the 561-594 laser line. Its excitation maximum is 662 nm.
- What filter should I use to detect RedDot 1?
- A 695/40 filter captures the peak of the RedDot 1 emission near 694 nm with minimal spillover.