CF680R
CF®680R (Ex-Max 680 nm/Em-Max 701 nm)
CF®680R from Biotium is a fluorophore with an excitation peak at 680 nm and an emission peak at 701 nm. It can be useful in both fluorescence microscopy and flow cytometry. For microscopy, CF®680R has been specifically validated in super-resolution imaging by STORM, STED and 2-Photon microscopy. It is spectrally similar to Alexa Fluor™ 680, IRDye® 680 and Cy5.5.
Excitation & emission spectra
Key facts
- Excitation maximum
- 680 nm
- Emission maximum
- 701 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
- Relative brightness
- 1 of 5
- Extinction coefficient
- 140000
- Molecular weight
- 912
- Commercial conjugates
- 30 from 1 supplier
CF680R has an excitation maximum at 680 nm and an emission maximum at 701 nm, a Stokes shift of 21 nm. Its extinction coefficient is 140000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is CF680R?
- CF®680R from Biotium is a fluorophore with an excitation peak at 680 nm and an emission peak at 701 nm. It can be useful in both fluorescence microscopy and flow cytometry. For microscopy, CF®680R has been specifically validated in super-resolution imaging by STORM, STED and 2-Photon microscopy. It is spectrally similar to Alexa Fluor™ 680, IRDye® 680 and Cy5.5.
- What laser excites CF680R?
- CF680R is optimally excited by the 628-640 laser line. Its excitation maximum is 680 nm.
- What filter should I use to detect CF680R?
- A 695/40 filter captures the peak of the CF680R emission near 701 nm with minimal spillover.
- How bright is CF680R?
- CF680R has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to CF680R?
- FluoroFinder tracks 30 commercially available CF680R conjugates, searchable by target, clone, host and application.