CF583
CF®583 (Ex-Max 583 nm/Em-Max 606 nm)
CF®583 from Biotium is a fluorophore with an excitation peak at 583 nm and an emission peak at 606 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Cy3.5.
Excitation & emission spectra
Key facts
- Excitation maximum
- 583 nm
- Emission maximum
- 606 nm
- Optimal laser line
- 561
- Optimal filter
- 610/20
- Relative brightness
- 2 of 5
- Extinction coefficient
- 150000
- Molecular weight
- 3127
- Commercial conjugates
- 4 from 1 supplier
CF583 has an excitation maximum at 583 nm and an emission maximum at 606 nm, a Stokes shift of 23 nm. Its extinction coefficient is 150000 M⁻¹cm⁻¹. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is CF583?
- CF®583 from Biotium is a fluorophore with an excitation peak at 583 nm and an emission peak at 606 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Cy3.5.
- What laser excites CF583?
- CF583 is optimally excited by the 561 laser line. Its excitation maximum is 583 nm.
- What filter should I use to detect CF583?
- A 610/20 filter captures the peak of the CF583 emission near 606 nm with minimal spillover.
- How bright is CF583?
- CF583 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to CF583?
- FluoroFinder tracks 4 commercially available CF583 conjugates, searchable by target, clone, host and application.