Vio 515
Vio®515 (Ex-Max 488 nm/Em-Max 514 nm)
Vio® 515 from Miltenyi has an excitation peak at 488 nm and an emission peak at 514. It is spectrally comparable to FITC, Alexa Fluor™ 488, CF®488A and DyLight™ 488.
Excitation & emission spectra
Key facts
- Excitation maximum
- 488 nm
- Emission maximum
- 514 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
- Relative brightness
- 2 of 5
- Commercial conjugates
- 53 from 1 supplier
Vio 515 has an excitation maximum at 488 nm and an emission maximum at 514 nm, a Stokes shift of 26 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Vio 515?
- Vio® 515 from Miltenyi has an excitation peak at 488 nm and an emission peak at 514. It is spectrally comparable to FITC, Alexa Fluor™ 488, CF®488A and DyLight™ 488.
- What laser excites Vio 515?
- Vio 515 is optimally excited by the 488 laser line. Its excitation maximum is 488 nm.
- What filter should I use to detect Vio 515?
- A 525/50 filter captures the peak of the Vio 515 emission near 514 nm with minimal spillover.
- How bright is Vio 515?
- Vio 515 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to Vio 515?
- FluoroFinder tracks 53 commercially available Vio 515 conjugates, searchable by target, clone, host and application.