Alexa Fluor 700
Alexa Fluor™ 700 (Ex-Max 702 nm/Em-Max 723 nm)
Alexa Fluor™ 700 (AF700, Alexa 700) has an excitation peak at 702 nm and an emission peak at 723 nm, and is spectrally similar to iFluor® 680 (ATT Bioquest) and iFluor® 700 (ATT Bioquest). Alexa 700 is commonly used in Flow Cytometry and is similar in size, brightness and application to iFluor® 700 and Vio® R720.
Excitation & emission spectra
Key facts
- Excitation maximum
- 702 nm
- Emission maximum
- 723 nm
- Optimal laser line
- 628-640
- Optimal filter
- 730/45
- Relative brightness
- 1 of 5
- Extinction coefficient
- 192000
- Commercial antibodies
- 25.3k from 18 suppliers
Alexa Fluor 700 has an excitation maximum at 702 nm and an emission maximum at 723 nm, a Stokes shift of 21 nm. Its extinction coefficient is 192000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Alexa Fluor 700?
- Alexa Fluor™ 700 (AF700, Alexa 700) has an excitation peak at 702 nm and an emission peak at 723 nm, and is spectrally similar to iFluor® 680 (ATT Bioquest) and iFluor® 700 (ATT Bioquest). Alexa 700 is commonly used in Flow Cytometry and is similar in size, brightness and application to iFluor® 700 and Vio® R720.
- What laser excites Alexa Fluor 700?
- Alexa Fluor 700 is optimally excited by the 628-640 laser line. Its excitation maximum is 702 nm.
- What filter should I use to detect Alexa Fluor 700?
- A 730/45 filter captures the peak of the Alexa Fluor 700 emission near 723 nm with minimal spillover.
- How bright is Alexa Fluor 700?
- Alexa Fluor 700 has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to Alexa Fluor 700?
- FluoroFinder tracks 25345 commercially available Alexa Fluor 700 conjugates, searchable by target, clone, host and application.