Duke University
DHVI Flow Cytometry and Cell Sorting Facility

Design a panel and find antibodies today at

https://app.fluorofinder.com/duke-dhvi
Logo: FluoroFinder

L01 - BD LSRII (RP105) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 Alexa Fluor 405
Brilliant Violet 421
eFluor 450
Live/Dead Fix Violet
Pacific Blue
V450
Zombie Violet
CF405M
DyLight 405
SuperBright 436

515 / 20

505

 AmCyan
Brilliant Violet 510
Live/Dead Fix Aqua
Pacific Orange
Qdot 525
V500
Zombie Aqua
Alexa Fluor 430
CF405L
CF430

560 / 40

555

 Brilliant Violet 570
eFluor 565NC
Qdot 565
Zombie Yellow
DyLight 405LS
Qdot 585

610 / 20

595

 Brilliant Violet 605
eFluor 605NC
Qdot 605
DyLight 405LS
eVolve 605
SuperBright 600

670 / 30

635

 Brilliant Violet 650
eFluor 650NC
Qdot 655
eVolve 655
SuperBright 645

710 / 50

695

 Brilliant Violet 711
Qdot 705
SuperBright 702

740 / 20

710

 Brilliant Violet 750

780 / 60

750

 Brilliant Violet 786
Qdot 800
Brilliant Violet 785
Laser

488

525 / 50

495

 Alexa Fluor 488
Brilliant Blue 515
CFSE
FITC
GFP
Zombie Green
ATTO 488
CF488A
CF503R
Cy2

695 / 40

685

 Brilliant Blue 700
PerCP-Cy5.5
PerCP-eFluor 710
PerCP-Vio700
Laser

532

575 / 26

550

 dsRed
dTomato
PE
CF550R
CF570

610 / 20

600

 Alexa Fluor 594
ECD
mCherry
PE-Alexa 610
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Texas Red
PE-Vio615
BODIPY TR

660 / 20

655

 PE-Cy5
7-AAD

710 / 50

695

 PE-Alexa 700
PE-Cy5.5

780 / 60

735

 PE-Cy7
PE-Vio770
Laser

640

670 / 30

--

 Alexa Fluor 647
APC
eFluor 660
Live/Dead Fix Far Red
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633

730 / 45

690

 Alexa Fluor 680
Alexa Fluor 700
APC-Cy5.5
APC-R700
Vybrant DyeCycle Ruby
DyLight 680

780 / 60

735

 APC-Cy7
APC-eFluor 780
APC-H7
APC-Alexa 750
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.