Duke University
DHVI Flow Cytometry and Cell Sorting Facility

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S01 - BD FACSymphony S6 (RTP 2104D) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

 Alexa Fluor 350
Brilliant Ultraviolet 395

450 / 50

410

 DAPI
Hoechst (34580)
DyLight 350

515 / 30

450

 Brilliant Ultraviolet 496

586 / 15

550

 Brilliant Ultraviolet 563

610 / 20

600

 Brilliant Ultraviolet 615

670 / 30

635

 Brilliant Ultraviolet 661
Hoechst (33342)

740 / 35

690

 Brilliant Ultraviolet 737

820 / 60

720

 Brilliant Ultraviolet 805
Laser

402

410 / 28

--

 Alexa Fluor 405
BFP
Brilliant Violet 421
CellTrace Violet
DyLight 405
eFluor 450
Live/Dead Fix Violet
Pacific Blue
Sytox Blue
Vio Blue
Vybrant DyeCycle Violet
Zombie Violet

470 / 14

450

 Alexa Fluor 430
AmCyan
Brilliant Violet 480
Cerulean
CFP
Live/Dead Fix Aqua
Pacific Green
Qdot 525
V500
Vio Green
Zombie Aqua

586 / 15

550

 Brilliant Violet 570
Live/Dead Fix Yellow
Pacific Orange
Qdot 565
Qdot 585
Alexa Fluor 430
Krome Orange

610 / 20

600

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

670 / 30

635

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

710 / 50

690

 Brilliant Violet 711
Qdot 705
SuperBright 702

750 / 30

740

 Brilliant Violet 750

780 / 60

750

 Brilliant Violet 786
Qdot 800
Brilliant Violet 785
Laser

488

515 / 20

505

 Alexa Fluor 488
Brilliant Blue 515
CFSE
Cy2
DyLight 488
Emerald GFP
FITC
GFP
Live/Dead Fix Green
Sytox Green
Vio Bright 515
Vybrant DyeCycle Green
YFP
Zombie Green

610 / 20

600

 Brilliant Blue 630-P2
PE-eFluor 610
PE-Texas Red
PE-Vio615

670 / 30

635

 Brilliant Blue 660-P2
PerCP

710 / 50

690

 Brilliant Blue 700
PerCP-Cy5.5
PerCP-eFluor 710
PerCP-Vio700
PE-Cy5.5

750 / 30

730

 Brilliant Blue 755-P

780 / 60

770

 Brilliant Blue 790-P
Laser

561

586 / 15

570

 Alexa Fluor 555
BYG584-P
CellTrace Yellow
Cy3
dsRed
eFluor 570
PE
RFP
Sytox Orange
tdTomato
Vybrant DyeCycle Orange

610 / 20

600

 ECD
mCherry
PE-CF594
PE-eFluor 610
PE-Texas Red
CF583
CF583R
DyLight 594
PE-Dazzle 594
PE-Vio615

670 / 30

650

 mPlum
PE-Cy5
Propidium Iodide
7-AAD

710 / 50

690

 PE-Alexa 700
PE-Cy5.5

780 / 60

750

 PE-Cy7
PE-Vio770
PE-Alexa 750
Laser

638

670 / 30

655

 Alexa Fluor 633
Alexa Fluor 635
Alexa Fluor 647
Alexa Fluor 680
APC
CellTrace Far Red
Cy5
Cy5.5
DyLight 633
Live/Dead Fix Far Red
Vybrant DyeCycle Ruby

710 / 50

690

 Alexa Fluor 700
APC-Cy5.5
APC-R700
Alexa Fluor 680
DyLight 680

780 / 60

750

 Alexa Fluor 750
APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-H7
Live/Dead Fix Near IR (780)
Zombie NIR
APC-Alexa 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.