Deutsches Rheuma-Forschungszentrum in Berlin (DRFZ)
FCCF

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BD FACS ARIA II "Floyd" Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 Alexa Fluor 405
Brilliant Violet 421
DAPI
Pacific Blue
V450
CF405M
DyLight 405
eFluor 450
SuperBright 436
Vio Blue

525 / 30

525

 Alexa Fluor 430
Brilliant Violet 510
Pacific Orange
V500
CF405L
CF430
CF440
DyLight 405LS
Krome Orange
Vio Green

610 / 20

600

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

710 / 50

685

 Brilliant Violet 711
Qdot 705
SuperBright 702
Laser

488

530 / 30

505

 Alexa Fluor 488
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

710 / 50

685

 PerCP-Cy5.5
Propidium Iodide
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

582 / 15

--

 PE
CF550R
CF568
CF570

610 / 20

600

 PE-Texas Red
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

660 / 20

635

 PE-Cy5

710 / 50

685

 PE-Cy5.5

780 / 60

750

 PE-Cy7
PE-Vio770
Laser

633

670 / 30

--

 Alexa Fluor 647
APC
Cy5
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cyanine 5 SE
DyLight 633
DyLight 650

780 / 60

750

 APC-Cy7
APC-H7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.