Dalhousie University
Faculty of Medicine Flow Cytometry Facility

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BD FACS Symphony A5 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

 Brilliant Ultraviolet 395

515 / 30

450

 Brilliant Ultraviolet 496

586 / 15

550

 Brilliant Ultraviolet 563

610 / 20

600

 Qdot 605
Qdot 625
eVolve 605

670 / 30

635

 Brilliant Ultraviolet 661

740 / 35

690

 Brilliant Ultraviolet 737

820 / 60

770

 Brilliant Ultraviolet 805
Laser

405

450 / 50

410

 Brilliant Violet 421
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
Pacific Blue
SuperBright 436
V450
Vio Blue

474 / 25

450

 Brilliant Violet 480

586 / 15

550

 Brilliant Violet 570
DyLight 405LS
Qdot 585

610 / 20

600

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

670 / 30

635

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

710 / 50

690

 Brilliant Violet 711
Qdot 705
SuperBright 702

740 / 35

710

 Brilliant Violet 750

780 / 60

750

 Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

505

 Brilliant Blue 515
FITC
Alexa Fluor 488
CF488A
CF503R
Cy2
DyLight 488
Fluorescein
Vio Bright FITC

610 / 20

600

 Brilliant Blue 630-P2

670 / 30

635

 Brilliant Blue 660-P2

710 / 50

685

 Brilliant Blue 700
PerCP-Cy5.5

780 / 60

750

 Brilliant Blue 790-P
Laser

561

586 / 15

--

 PE
CF550R
CF568
CF570

610 / 20

600

 PE-CF594
DyLight 594
ECD
PE-Dazzle 594
PE-Texas Red
PE-Vio615

670 / 30

635

 PE-Cy5

710 / 50

685

 PE-Cy5.5

780 / 60

750

 PE-Cy7
PE-Vio770
Laser

628

670 / 30

--

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
Cy5
DyLight 633
DyLight 650
NL 637

730 / 45

690

 APC-R700
Alexa Fluor 700

780 / 60

750

 APC-H7
APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.