Dalhousie University
Faculty of Medicine Flow Cytometry Facility

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FACSCanto II Configuration

Laser (nm) BP SP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

--

Pacific Blue
Alexa Fluor 405
Brilliant Violet 421
CF405M
DyLight 405
eFluor 450
V450
Vio Blue

510 / 50

--

502

AmCyan
Alexa Fluor 430
Brilliant Violet 510
V500
Vio Green
Laser

488

530 / 30

--

502

FITC
Alexa Fluor 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein
Vio Bright FITC

585 / 42

--

556

PE

--

735

670

PerCP
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

780 / 60

--

735

PE-Cy7
PE-Vio770
Laser

633

660 / 20

--

--

APC
Alexa Fluor 647
CF640R
CF647
Cy5
DyLight 633
NL 637

780 / 60

--

735

APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.