Dalhousie University
Faculty of Medicine Flow Cytometry Facility

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Fortessa Full Power Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

440 / 40

--

 BFP
Brilliant Violet 421
DAPI
eFluor 450
V450
Alexa Fluor 405
CF405M
DyLight 405
Pacific Blue
SuperBright 436

525 / 50

505

 AmCyan
eFluor 506 Fix Viability
Alexa Fluor 430
Brilliant Violet 510
V500
Vio Green

575 / 26

550

 Brilliant Violet 570
DyLight 405LS
Qdot 565
Qdot 585

605 / 19

595

 Brilliant Violet 605
eFluor 625NC
eVolve 605
DyLight 405LS
eFluor 605NC
Qdot 605
SuperBright 600

660 / 20

640

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

710 / 40

685

 Brilliant Violet 711
Qdot 705
SuperBright 702

750 / 30

735

 Brilliant Violet 750

783 / 40

760

 Brilliant Violet 785
Brilliant Violet 786
Qdot 800
Laser

488

515 / 20

505

 Alexa Fluor 488
CFSE
FITC
GFP
Oregon Green 488
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

710 / 50

685

 PerCP
PerCP-Cy5.5
PerCP-eFluor 710
Brilliant Blue 700
PerCP-Vio700
Laser

532

575 / 25

--

 Alexa Fluor 532
Cy3
PE
Alexa Fluor 546
Alexa Fluor 555
ATTO 532
ATTO 532 Phalloidin
CF550R
CF570
Cyanine 3 SE

610 / 20

600

 mCherry
PE-eFluor 610
PE-Texas Red
Propidium Iodide
7-AAD
ECD
PE-CF594
PE-Dazzle 594
PE-Vio615

660 / 40

640

 PE-Alexa 647
PE-Cy5
7-AAD

710 / 50

690

 PE-Alexa 700
PE-Cy5.5

780 / 40

740

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

628

670 / 30

--

 Alexa Fluor 647
APC
eFluor 660
Alexa Fluor 633
Alexa Fluor 635
CF640R
Cy5
DyLight 633
DyLight 650
NL 637

730 / 45

685

 Alexa Fluor 660
Alexa Fluor 680
Alexa Fluor 700
APC-Cy5.5
iRFP
APC-R700
DyLight 680

780 / 60

740

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.