Dalhousie University
Faculty of Medicine Flow Cytometry Facility

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FACSAria III Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

488

530 / 30

502

 Alexa Fluor 488
CFSE
FITC
GFP
Oregon Green 488
YFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R

585 / 15

556

 dsRed
PE
RFP
tdTomato

616 / 23

610

 PE-eFluor 610
PE-Texas Red
Propidium Iodide
7-AAD
ECD
PE-CF594
PE-Dazzle 594
PE-Vio615

695 / 40

655

 PE-Cy5
PE-Cy5.5
PerCP
PerCP-Cy5.5
PerCP-eFluor 710
Brilliant Blue 700
PerCP-Vio700

780 / 60

735

 PE-Cy7
PE-Vio770
Laser

633

660 / 20

--

 Alexa Fluor 647
APC
eFluor 660
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650

730 / 45

690

 Alexa Fluor 700
iRFP
APC-R700

780 / 60

735

 APC-Cy7
APC-eFluor 780
APC-H7
APC-Alexa 750
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.