Spanish National Center for Cardiovascular Research (CNIC)
Flow Cytometry Unit

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BD Symphony A5 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

370

 Brilliant Ultraviolet 395

450 / 50

410

 Alexa Fluor 350
DAPI
CF350
DyLight 350

515 / 30

495

 Brilliant Ultraviolet 496
Live/Dead Fix Aqua

580 / 20

550

 Brilliant Ultraviolet 563

605 / 20

595

 Brilliant Ultraviolet 615-P
Propidium Iodide

670 / 25

630

 Brilliant Ultraviolet 661

735 / 30

690

 Brilliant Ultraviolet 737

810 / 40

770

 Brilliant Ultraviolet 805
Laser

405

431 / 28

410

 Alexa Fluor 405
Brilliant Violet 421
eFluor 450
Pacific Blue
V450
CF405M
DyLight 405
SuperBright 436
Vio Blue

525 / 50

505

 Brilliant Violet 510
eFluor 506
Pacific Green
V500
Alexa Fluor 430
AmCyan
CF405L
CF430
CF440
DY-380XL

586 / 15

550

 Brilliant Violet 570
Pacific Orange
Alexa Fluor 430
DyLight 405LS
Krome Orange
Qdot 585

605 / 40

595

 Brilliant Violet 605
eVolve 605
DyLight 405LS
eFluor 605NC
Qdot 605
SuperBright 600

677 / 20

635

 Brilliant Violet 650
eVolve 655
eFluor 650NC

710 / 50

685

 Brilliant Violet 711
Qdot 705
SuperBright 702

810 / 40

770

 Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

505

 Alexa Fluor 488
Brilliant Blue 515
CFSE
FITC
GFP
Sytox Green
YFP
ATTO 488
CF488A
CF503R

610 / 20

600

 Brilliant Blue 630-P2
Propidium Iodide

670 / 30

635

 Brilliant Blue 660-P2
PerCP

710 / 50

685

 PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

750 / 30

735

 Brilliant Blue 790-P
PE-Vio770
PE-Alexa 750
Laser

561

586 / 15

570

 Cy3
dsRed
dTomato
eFluor 570
PE
RFP
Alexa Fluor 546
Alexa Fluor 555
CF550R
CF568

610 / 20

600

 mCherry
PE-CF594
PE-Dazzle 594
PE-Texas Red
CF583
CF583R
DyLight 594
ECD
PE-eFluor 610
PE-Vio615

670 / 30

635

 PE-Cy5
7-AAD

710 / 50

685

 PE-Cy5.5

780 / 60

750

 PE-Cy7
PE-Vio770
PE-Alexa 750
Laser

637

670 / 30

655

 Alexa Fluor 647
APC
Cy5
DRAQ 5
eFluor 660
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cyanine 5 SE

730 / 45

690

 Alexa Fluor 700
APC-Cy5.5
APC-R700
Alexa Fluor 680
DyLight 680

780 / 60

750

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.