Spanish National Center for Cardiovascular Research (CNIC)
Flow Cytometry Unit

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FACSCantoII 3L Configuration

Laser (nm) BP SP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

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--

 Alexa Fluor 405
Brilliant Violet 421
DAPI
eFluor 450
Pacific Blue
V450
CF405M
DyLight 405
SuperBright 436
Vio Blue

510 / 50

--

502

 AmCyan
Brilliant Violet 510
Pacific Orange
V500
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
Krome Orange
Laser

488

530 / 30

--

520

 Alexa Fluor 488
CFSE
FITC
GFP
Venus
YFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R

585 / 42

--

556

 PE
Propidium Iodide

--

735

670

 Brilliant Blue 700
PE-Cy5
PE-Cy5.5
PerCP
PerCP-Cy5.5
PerCP-eFluor 710
7-AAD
PerCP-Vio700

780 / 60

--

--

 PE-Cy7
PE-Vio770
Laser

633

660 / 20

--

--

 Alexa Fluor 647
APC
Cy5
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cyanine 5 SE
DyLight 633
DyLight 650

780 / 60

--

735

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.