University of Florida
Center for Immunology and Transplantation

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ImageStream X Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

467 / 75

--

 Alexa Fluor 405
Cascade Blue
CFP
DAPI
DyLight 405
eFluor 450
Hoechst (33258)
Live/Dead Fix Violet
Pacific Blue
Brilliant Violet 421

537 / 65

--

 Alexa Fluor 430
Cascade Yellow
Pacific Orange
Qdot 525
Brilliant Violet 510
Brilliant Violet 570
DyLight 405LS
Krome Orange
V500
Vio Green

627 / 65

--

 eFluor 625NC
Qdot 625
Brilliant Violet 605

702 / 85

--

 eFluor 650NC
Qdot 705
Brilliant Violet 650
eVolve 655
Qdot 655

772 / 55

--

 Qdot 800
Brilliant Violet 785
Brilliant Violet 786
Laser

488

520 / 80

--

 Alexa Fluor 488
DyLight 488
FITC
GFP
LysoTracker Green
MitoTracker Green
PKH67
Qdot 525
YFP
ATTO 488

577 / 35

--

 Alexa Fluor 555
Cy3
dsRed
PE
PKH26
Cyanine 3 SE

627 / 65

--

 ECD
eFluor 625NC
PE-Alexa 610
PE-Texas Red
Propidium Iodide
Qdot 625
RFP
7-AAD
PE-CF594
PE-Dazzle 594

702 / 85

--

 DRAQ 5
eFluor 650NC
PE-Alexa 647
PE-Cy5
PerCP
PerCP-Cy5.5
Qdot 705
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

772 / 55

--

 PE-Alexa 750
PE-Cy7
Qdot 800
PE-Vio770
Laser

658

702 / 85

--

 Alexa Fluor 647
Alexa Fluor 660
Alexa Fluor 680
APC
Cy5
DRAQ 5
DyLight 649
DyLight 680
PE-Alexa 647
PE-Cy5
PerCP
PerCP-Cy5.5

772 / 55

--

 Alexa Fluor 750
APC-Alexa 750
APC-Cy7
APC-eFluor 780
Cy7
DyLight 750
PE-Alexa 750
PE-Cy7
APC-H7

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.