Columbia University
Columbia CCTI/HICCC flow cytometry core facility

Design a panel and find antibodies today at

https://app.fluorofinder.com/ccti-columbia
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CCTI Fortessa Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 Brilliant Violet 421
DAPI
Live/Dead Fix Violet
Pacific Blue
SuperBright 436
Sytox Blue
V450
Alexa Fluor 405
CF405M
DyLight 405

525 / 50

505

 Brilliant Violet 510
CFP
Live/Dead Fix Aqua
Sytox Blue
V500
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS

610 / 20

595

 Brilliant Violet 605
SuperBright 600
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605

660 / 20

630

 Brilliant Violet 650
SuperBright 645
eFluor 650NC
eVolve 655
Qdot 655

710 / 50

690

 Brilliant Violet 711
SuperBright 702
Qdot 705

780 / 60

750

 Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

505

 Alexa Fluor 488
FITC
GFP
Live/Dead Fix Green
Sytox Green
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

575 / 26

550

 PE

610 / 20

600

 PE-Texas Red
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

710 / 50

685

 PE-Cy5
PerCP
PerCP-Cy5.5
Propidium Iodide
7-AAD
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

780 / 60

750

 PE-Cy7
PE-Vio770
Laser

633

670 / 14

--

 Alexa Fluor 633
Alexa Fluor 647
APC
DRAQ 5
Live/Dead Fix Far Red
Sytox Red
Alexa Fluor 635
CF640R
CF647
Cy5

730 / 45

710

 Alexa Fluor 700
APC-R700

780 / 60

750

 APC-Cy7
APC-H7
Live/Dead Fix Near IR (775)
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.