Columbia University
Columbia CCTI/HICCC flow cytometry core facility

Design a panel and find antibodies today at

https://app.fluorofinder.com/ccti-columbia
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CCTI Influx Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

460 / 50

--

Brilliant Violet 421
DAPI
Live/Dead Fix Violet
Pacific Blue
Sytox Blue
V450
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450

520 / 34

--

AmCyan
Brilliant Violet 510
Live/Dead Fix Aqua
Pacific Orange
Qdot 525
Sytox Blue
V500
Alexa Fluor 430
CF405L
CF430

610 / 20

--

Brilliant Violet 605
Qdot 605
DyLight 405LS
eFluor 605NC
eVolve 605
SuperBright 600

660 / 20

--

Brilliant Violet 650
Qdot 655
eFluor 650NC
eVolve 655
SuperBright 645

710 / 50

--

Brilliant Violet 711
Qdot 705
SuperBright 702

--

750

Brilliant Violet 785
Qdot 800
Brilliant Violet 786
Laser

488

530 / 40

--

Alexa Fluor 488
FITC
GFP
Live/Dead Fix Green
Sytox Green
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

692 / 40

--

PerCP-Cy5.5
Propidium Iodide
7-AAD
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

585 / 28

--

PE
Sytox Orange
CF550R
CF568
CF570

610 / 20

--

Live/Dead Fix Red
PE-Texas Red
Propidium Iodide
7-AAD
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Dazzle 594

670 / 30

--

PE-Cy5
Propidium Iodide
7-AAD

--

750

PE-Cy7
PE-Vio770
Laser

638

670 / 30

--

Alexa Fluor 633
Alexa Fluor 647
APC
Live/Dead Fix Far Red
Sytox Red
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633

720 / 40

--

Alexa Fluor 700
APC-R700

--

750

APC-Cy7
APC-H7
Live/Dead Fix Near IR (775)
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.