Columbia University
Columbia CCTI/HICCC flow cytometry core facility

Design a panel and find antibodies today at

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CCTI LSR II Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

 Alexa Fluor 350
DAPI
Hoechst (33258)
Indo-Hi (Ca2+bound)
Live/Dead Fix Blue
CF350
DyLight 350

515 / 30

505

 Brilliant Ultraviolet 496
Indo-Lo (Ca2+free)
Live/Dead Fix Aqua
Qdot 525

620 / 10

610

 Hoechst (33342)
Qdot 605
eVolve 605
Laser

405

450 / 50

--

 Alexa Fluor 405
Brilliant Violet 421
Cascade Blue
DAPI
Live/Dead Fix Violet
Pacific Blue
Sytox Blue
CF405M
DyLight 405
eFluor 450

515 / 30

505

 Alexa Fluor 430
AmCyan
Brilliant Violet 510
CFP
Live/Dead Fix Aqua
Pacific Orange
Sytox Blue
CF405L
CF430
CF440

610 / 20

595

 Brilliant Violet 605
Qdot 605
DyLight 405LS
eFluor 605NC
eVolve 605
SuperBright 600

655 / 12

635

 Brilliant Violet 650
Qdot 655
eFluor 650NC
eVolve 655
SuperBright 645

710 / 50

685

 Brilliant Violet 711
Qdot 705
SuperBright 702
Laser

488

525 / 50

505

 Alexa Fluor 488
FITC
GFP
Live/Dead Fix Green
Sytox Green
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

710 / 50

685

 PE-Cy5
PerCP-Cy5.5
Propidium Iodide
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

532

575 / 24

--

 PE
Sytox Orange
CF550R
CF570

610 / 20

600

 Live/Dead Fix Red
PE-CF594
PE-Texas Red
Propidium Iodide
7-AAD
ECD
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

780 / 60

750

 PE-Cy7
PE-Vio770
Laser

594

620 / 20

--

 Alexa Fluor 568
Alexa Fluor 594
mCherry
mStrawberry
Texas Red
ATTO 594

685 / 34

670

 mPlum
Laser

633

670 / 30

--

 Alexa Fluor 633
Alexa Fluor 647
APC
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
NL 637

730 / 44

685

 Alexa Fluor 700
APC-R700

780 / 60

750

 APC-Cy7
APC-eFluor 780
APC-H7
APC-Alexa 750
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.