Cincinnati Children's
Research Flow Cytometry Core Facility

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Bigfoot 5L Spectral (12UV, 12V, 7B, 12YG, 5R) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

349

387 / 11

--

 Brilliant Ultraviolet 395

420 / 10

--

 Alexa Fluor 350
CF350

434 / 17

--

 AMCA

455 / 14

--

 DAPI
DyLight 350

473 / 15

--

 Live/Dead Fix Blue

507 / 19

--

 Brilliant Ultraviolet 496

549 / 15

--

 Brilliant Ultraviolet 563

575 / 15

--

 Brilliant Ultraviolet 563

615 / 24

--

 Brilliant Ultraviolet 615

670 / 30

--

 Brilliant Ultraviolet 661

728 / 40

--

 Brilliant Ultraviolet 737

--

750

 Brilliant Ultraviolet 805
Laser

405

420 / 10

--

 Brilliant Violet 421
Alexa Fluor 405
DyLight 405
SuperBright 436
Vio Blue

434 / 17

--

 SuperBright 436

455 / 14

--

 Pacific Blue
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
V450
Vio Blue

473 / 15

--

 Brilliant Violet 480

507 / 19

--

 Brilliant Violet 510
Alexa Fluor 430
CF405L
CF430
CF440
V500
Vio Green

549 / 15

--

 Live/Dead Fix Yellow

575 / 15

--

 Brilliant Violet 570
DyLight 405LS
Qdot 565
Qdot 585

615 / 24

--

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

661 / 20

--

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

710 / 20

--

 Brilliant Violet 711
Qdot 705
SuperBright 702

747 / 33

--

 Brilliant Violet 750

--

770

 Brilliant Violet 785
Qdot 800
Laser

488

507 / 19

--

 FITC
Alexa Fluor 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein
Vio Bright FITC

549 / 15

--

 NovaFluor Blue 555

583 / 30

--

 NovaFluor Blue 585

615 / 24

--

 NovaFluor Blue 610 / 30S

670 / 30

--

 PerCP

720 / 60

--

 PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

--

750

 Brilliant Blue 755-P
Brilliant Blue 790-P
cFluor BYG781
Laser

561

575 / 15

--

 Alexa Fluor 546
Spark YG 581
Alexa Fluor 555
ATTO 550
Cy3
DyLight 549
DyLight 550
Dyomics 547
eFluor 570
NL 557

589 / 15

--

 Alexa Fluor 568
Cy3.5
Dyomics 590
Rhodamine Red
Rhodamine Red-X

605 / 15

--

 PE-Dazzle 594
DyLight 594
ECD
PE-CF594
PE-Texas Red
PE-Vio615

625 / 15

--

 ATTO Rho3B
BactoView Live Red
BOBO-3
CF570
cFluor BYG610
cFluor YG610
LipidSpot 610
LysoBrite Red
LysoView 594
Magic Red
MitoLite Red FX600
MitoMark Red
mNeptune
mScarlet
NovaFluor Yellow 610
PE-Alexa 610
PE-ATTO 594
PE-CF594
PE-Dazzle 594
PE-DyLight 594
PE-Dyomics 590
PE-eFluor 610
PE-Fire 640
PE-Vio615
PhenoVue 578 Mitochondrial Stain
PhenoVue Nile Red Lipid Stain
ReAsH
SNARF 1 pH6.0
SNARF 1 pH9.0
SureLight PE-594
SureLight RPC

685 / 15

--

 PE-Cy5.5

700 / 13

--

 NovaFluor Yellow 700

720 / 24

--

 PE-Alexa 700

760 / 50

--

 PE-Cy7
PE-Vio770

800 / 12

--

 PE-Alexa 750
PE-Vio770

832 / 37

--

 PE-Fire 810

--

860
Laser

640

670 / 30

--

 APC
Alexa Fluor 647
CF640R
CF647
Cy5
DyLight 633
NL 637

700 / 13

--

 APC-Alexa 700

720 / 24

--

 APC-Cy5.5
Alexa Fluor 680
DyLight 680

760 / 50

--

 APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

--

770

 APC-eFluor 780
APC-Fire 810
APC-Alexa 750
APC-Cy7
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.