Cincinnati Children's
Research Flow Cytometry Core Facility

Design a panel and find antibodies today at

https://app.fluorofinder.com/cchmc
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Fortessa 3 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

 DAPI
Hoechst (33342)
Live/Dead Fix Blue
Zombie UV
DyLight 350

525 / 50

505

 Indo-Lo (Ca2+free)
Laser

405

450 / 50

--

 Alexa Fluor 405
Brilliant Violet 421
eFluor 450
Pacific Blue
V450
CF405M
DyLight 405
SuperBright 436
Vio Blue

525 / 50

505

 AmCyan
Brilliant Violet 510
V500
Vio Green
Alexa Fluor 430
Cascade Yellow
CF405L
CF430
CF440
DyLight 405LS

610 / 20

600

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

670 / 30

635

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

710 / 50

690

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

750

 Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

505

 Alexa Fluor 488
Brilliant Blue 515
FITC
GFP
Venus
YFP
ATTO 488
CF488A
CF503R
Cy2

695 / 40

685

 PerCP
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

586 / 15

--

 dsRed
dTomato
PE
CF550R
CF568
CF570

610 / 20

600

 mCherry
PE-Alexa 610
PE-CF594
Propidium Iodide
DyLight 594
ECD
PE-Dazzle 594
PE-Texas Red
PE-Vio615

660 / 20

635

 PE-Alexa 647
PE-Cy5
7-AAD

710 / 50

685

 PE-Cy5.5

780 / 60

750

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

640

670 / 14

--

 Alexa Fluor 633
Alexa Fluor 647
APC
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
Dyomics 647

710 / 50

690

 Alexa Fluor 700
APC-Cy5.5
Alexa Fluor 680
APC-R700
DyLight 680

780 / 60

750

 APC-Alexa 750
APC-Cy7
APC-H7
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.