University of Calgary
University of Calgary Flow Cytometry Core Facility

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SH800 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 Alexa Fluor 405
Brilliant Violet 421
CFP
DAPI
Hoechst (33342)
Pacific Blue
CF405M
DyLight 405
eFluor 450
SuperBright 436

525 / 50

--

 AmCyan
Brilliant Violet 510
Pacific Orange
Qdot 525
Qdot 545
T-Sapphire
Alexa Fluor 430
CF405L
CF430
CF440

600 / 60

--

 Brilliant Violet 570
Qdot 585
Qdot 605
Brilliant Violet 605
DyLight 405LS
eVolve 605
Qdot 565

665 / 30

--

 Qdot 655
Brilliant Violet 650
eVolve 655

720 / 60

--

 Qdot 705

785 / 60

--

 Qdot 800
Brilliant Violet 785
Brilliant Violet 786
Laser

488

525 / 50

--

 Alexa Fluor 488
CFSE
eGFP
eYFP
FITC
mCitrine
ATTO 488
Brilliant Blue 515
CF488A
CF503R

600 / 60

--

 PE
PE-Texas Red
Propidium Iodide
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

665 / 30

--

 PE-Cy5
PerCP
7-AAD
PerCP-Vio700

720 / 60

--

 PE-Cy5.5
PerCP-Cy5.5
PerCP-eFluor 710
Brilliant Blue 700
PerCP-Vio700

785 / 60

--

 PE-Cy7
PE-Vio770
Laser

561

600 / 60

--

 dsRed
mOrange
tdTomato

665 / 30

--

 mCherry
mPlum

720 / 60

--

 PE-Alexa 700

785 / 60

--

 PE-Cy7
PE-Vio770
Laser

638

665 / 30

--

 Alexa Fluor 647
APC
Cy5
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cyanine 5 SE
DyLight 633
DyLight 650

720 / 60

--

 Alexa Fluor 700
APC-Cy5.5
Alexa Fluor 680
APC-R700
DyLight 680

785 / 60

--

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.