University of Calgary
University of Calgary Flow Cytometry Core Facility

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CytoFLEX Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

375

450 / 45

--

Brilliant Ultraviolet 395
DAPI

525 / 40

--

Brilliant Ultraviolet 496

675 / 30

--

Brilliant Ultraviolet 661
Laser

405

450 / 45

--

Brilliant Violet 421
eFluor 450
Pacific Blue
V450
Alexa Fluor 405
CF405M
DyLight 405
SuperBright 436
Vio Blue

525 / 40

--

AmCyan
Brilliant Violet 510
Krome Orange
V500
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
Vio Green

610 / 20

--

Brilliant Violet 605
Qdot 605
DyLight 405LS
eFluor 605NC
eVolve 605
SuperBright 600

660 / 10

--

Brilliant Violet 650
Qdot 655
eFluor 650NC
eVolve 655
SuperBright 645

763 / 43

--

Brilliant Violet 785
Qdot 800
Brilliant Violet 786
Laser

488

525 / 40

--

Alexa Fluor 488
CFSE
FITC
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

610 / 20

--

ECD
PE-CF594
PE-Texas Red
Propidium Iodide
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

690 / 50

--

PE-Cy5
PE-Cy5.5
PerCP
PerCP-Cy5.5
Propidium Iodide
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

585 / 42

--

dsRed
PE
CF550R
CF568
CF570

610 / 20

--

ECD
mCherry
PE-CF594
DyLight 594
PE-Dazzle 594
PE-Texas Red
PE-Vio615

675 / 30

--

mPlum
PE-Cy5

710 / 50

--

PE-Cy5.5

763 / 43

--

PE-Cy7
PE-Vio770
Laser

638

660 / 10

--

Alexa Fluor 647
APC
Cy5
eFluor 660
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cyanine 5 SE
DyLight 633

712 / 25

--

Alexa Fluor 700
APC-R700
Cy5.5

763 / 43

--

APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.