Brown University
Brown Flow Cytometry and Sorting Facility

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Aria IIIu Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

407

450 / 50

--

 Brilliant Violet 421
DAPI
eFluor 450
Pacific Blue
Alexa Fluor 405
CF405M
DyLight 405
SuperBright 436
V450
Vio Blue

530 / 30

502

 Alexa Fluor 430
Brilliant Violet 510
Pacific Orange
V500
CF405L
CF430
CF440
DyLight 405LS
Krome Orange
Vio Green

582 / 15

530

 Brilliant Violet 570
DyLight 405LS
Qdot 585

605 / 40

595

 Brilliant Violet 605
eFluor 605NC
Qdot 605
DyLight 405LS
eVolve 605
SuperBright 600

660 / 40

630

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

710 / 50

690

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

750

 Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

502

 Alexa Fluor 488
CFSE
FITC
GFP
Sytox Green
YFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R

695 / 40

655

 PerCP
PerCP-Cy5.5
PerCP-eFluor 710
Brilliant Blue 700
PerCP-Vio700
Laser

561

585 / 42

556

 Alexa Fluor 555
PE
tdTomato
CF550R
CF568
CF570
Cy3

616 / 23

610

 dsRed
mCherry
PE-Texas Red
Propidium Iodide
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Dazzle 594

670 / 14

630

 PE-Cy5
7-AAD

710 / 40

680

 PE-Cy5.5

780 / 60

735

 PE-Cy7
PE-Vio770
Laser

633

660 / 20

--

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
NL 637

730 / 45

685

 APC-Alexa 680
APC-Alexa 700
DRAQ 5

780 / 60

735

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.