Brigham and Women's Hospital
Center for Cellular Profiling

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LSR Fortessa - BTM6006F Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

 Indo-Hi (Ca2+bound)

530 / 30

505

 Indo-Lo (Ca2+free)
Laser

405

450 / 40

--

 Brilliant Violet 421
DAPI
Pacific Blue
V450
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
SuperBright 436
Vio Blue

535 / 50

505

 AmCyan
Brilliant Violet 510
V500
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
Vio Green

610 / 20

595

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

660 / 20

630

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

710 / 50

690

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

750

 Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

505

 Brilliant Blue 515
FITC
Alexa Fluor 488
CF488A
CF503R
Cy2
DyLight 488
Fluorescein
Vio Bright FITC

695 / 40

685

 PE-Cy5.5
PerCP
PerCP-Vio700
Laser

561

575 / 26

550

 PE
CF550R
CF568
CF570

610 / 20

600

 PE-Texas Red
Propidium Iodide
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

670 / 30

655

 PE-Cy5

780 / 60

750

 PE-Cy7
PE-Vio770
Laser

640

670 / 14

--

 APC
Alexa Fluor 647
CF640R
CF647
Cy5
DyLight 633
NL 637

730 / 45

710

 Alexa Fluor 700
APC-R700

780 / 60

750

 APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.