Baylor College of Medicine
BCM Cytometry and Cell Sorting Facility

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LSR II (M902) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

 Brilliant Ultraviolet 395

450 / 50

410

 Alexa Fluor 350
DAPI
Hoechst (33258)
CF350
DyLight 350

--

650

 Hoechst (33342)
Laser

407

440 / 40

--

 Alexa Fluor 405
Brilliant Violet 421
Pacific Blue
SuperBright 436
CF405M
DyLight 405
eFluor 450
V450
Vio Blue

530 / 30

505

 Alexa Fluor 430
AmCyan
Brilliant Violet 510
CF405L
CF430
CF440
DyLight 405LS
Krome Orange
Pacific Orange
V500

610 / 20

595

 Brilliant Violet 605
Qdot 585
Qdot 625
SuperBright 600
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605

670 / 30

630

 Brilliant Violet 650
Qdot 655
SuperBright 645
eFluor 650NC
eVolve 655
Laser

488

530 / 30

505

 Alexa Fluor 488
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

575 / 26

550

 dsRed
PE
Pyronin Y

695 / 40

685

 PerCP
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

780 / 60

735

 PE-Cy7
PE-Vio770
Laser

633

660 / 20

--

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
NL 637

730 / 45

685

 Alexa Fluor 700
Vybrant DyeCycle Ruby
APC-R700

780 / 60

735

 Alexa Fluor 750
APC-Cy7
APC-H7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.