Baylor College of Medicine
BCM Cytometry and Cell Sorting Facility

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Aria-II Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

 Alexa Fluor 350
DAPI
Hoechst (33258)
CF350
DyLight 350

530 / 30

505

 Brilliant Ultraviolet 496
Brilliant Violet 510
Calcein Blue AM
Laser

405

450 / 50

--

 Alexa Fluor 405
Brilliant Violet 421
Pacific Blue
SuperBright 436
CF405M
DyLight 405
eFluor 450
V450
Vio Blue

525 / 50

505

 Alexa Fluor 430
AmCyan
Brilliant Violet 510
CF405L
CF430
CF440
DyLight 405LS
Krome Orange
Pacific Orange
V500

670 / 30

630

 Brilliant Violet 650
Qdot 655
SuperBright 645
eFluor 650NC
eVolve 655
Laser

488

530 / 30

505

 Alexa Fluor 488
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

675 / 20

670

 PerCP
Propidium Iodide
Laser

561

585 / 15

570

 Alexa Fluor 546
Cy3
dsRed
PE
Alexa Fluor 555
ATTO 550
CF550R
CF568
CF570
Cyanine 3 SE

610 / 20

600

 mCherry
PE-Texas Red
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

660 / 20

635

 PE-Cy5

710 / 50

685

 PE-Alexa 700
PE-Cy5.5

780 / 60

755

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

640

660 / 20

--

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
Dyomics 647

730 / 45

690

 Alexa Fluor 700
Vybrant DyeCycle Ruby
APC-R700

780 / 60

755

 Alexa Fluor 750
APC-Cy7
APC-H7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.