Baylor College of Medicine
BCM Cytometry and Cell Sorting Facility

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Amnis ImageStreamX Mark II Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

457 / 45

--

 Brilliant Violet 421
Cascade Blue
CFP
DAPI
DyLight 405
eFluor 450
Hoechst (33342)
Live/Dead Fix Violet
Pacific Blue
Alexa Fluor 405

537 / 65

--

 Alexa Fluor 430
Cascade Yellow
Pacific Orange
Qdot 525
Brilliant Violet 510
Brilliant Violet 570
DyLight 405LS
Krome Orange
V500
Vio Green

582 / 25

--

 Qdot 565
Qdot 585
Brilliant Violet 570

610 / 30

--

 Brilliant Violet 605
eFluor 625NC
Qdot 625
SuperBright 600
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605

702 / 85

--

 Brilliant Violet 711
Qdot 705
SuperBright 702

762 / 35

--

 Brilliant Violet 786
Qdot 800
Brilliant Violet 785
Laser

488

528 / 65

--

 Alexa Fluor 488
DyLight 488
FITC
LysoTracker Green
MitoTracker Green
Spectrum Green
ATTO 488
Brilliant Blue 515
CF488A
CF503R

577 / 35

--

 dsRed
PE
PKH26
Sytox Orange

610 / 30

--

 ECD
eFluor 625NC
PE-Alexa 610
PE-Texas Red
Qdot 625
RFP
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

702 / 85

--

 DRAQ 5
eFluor 650NC
Fura Red AM (Ca2+bound)
LDS 751
PE-Alexa 647
PE-Cy5
PerCP
PerCP-Cy5.5
Propidium Iodide
7-AAD

762 / 35

--

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

561

577 / 35

--

 Alexa Fluor 546
Cy3
dsRed
DyLight 550
PE
PKH26
Alexa Fluor 555
ATTO 550
CF550R
CF568

610 / 30

--

 Alexa Fluor 568
DyLight 594
ECD
mCherry
PE-Alexa 610
PE-Texas Red
RFP
CF583
CF583R
Cy3.5

702 / 85

--

 DRAQ 5
LDS 751
PE-Alexa 647
PE-Cy5
7-AAD

762 / 35

--

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

642

702 / 85

--

 Alexa Fluor 647
Alexa Fluor 660
Alexa Fluor 680
APC
Cy5
DRAQ 5
DyLight 649
DyLight 680
PE-Alexa 647
PE-Cy5
PerCP
PerCP-Cy5.5

762 / 35

--

 Alexa Fluor 750
APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
Cy7
DyLight 750
PE-Alexa 750
PE-Cy7
APC-Fire 750

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.